Wednesday, 17 August 2016

Cancer Cell Lines: MHC Class 1-Related Chain A and B Ligands Differently Expressed

In 1994, a new set off loci related to MHC class 1 genes called MHC class 1 chain-related genes (MIC) or Perth beta block transcript 11 were identified independently by Bahram et al. and Leelayuwat et al.in which five copies existed. The nomenclature was then standardized as MIC, which since then is in current use. Major Histocompatibility complex class 1chain related molecule is known to play an important role in tumor immune-surveillance.


Cancer Cell Lines

MICA/B is encoded in the MHC (major histocompatibility complex) region, and they share structural and sequence similarity with MHC class I proteins (28-35%). MICA/B has α1-α2-α3 extracellular domains and short transmembrane tails similar to MHC class 1. MICA/B does not associate with β2-microglobulin or antigenic peptides like their counterpart MHC class 1. They are highly polymorphic, with close to 60 recognizable MICA and 25 MICB alleles. Although much is not known about the significance of their polymorphism, MICA alleles might vary in their affinity for NKG2D binding and thus affect the thresholds of recognition by NK cells and T lymphocytes.

The MIC molecules have been detected in broader range of tumorshematological malignances and various adenocarcinomas such as breast, lung, colon, kidney, ovary and prostate tumors, gliomas, neuroblastomas and melanomas. Previous studies show that MIC genes are widely and transcribed and therefore possibly translated and membrane bound with exception of the Central Nervous system.

In human, NKG2D recognizes two structurally distinct families of ligands namely (MIC) molecules and the UL 16 binding proteins (ULBPs) 1-5 molecules which is also known as RAET1: retinoic acid early transcript, originally identified through interaction with Cytomegalovirus UL 16 glycoproteins. MIC and ULBP both engage NKG2D, which then triggers cytokine production and cytotoxic activity seen in activated NK cell.

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