Jatropha Curcas Linn can Reduce the Expression of Hsp70 that will Result in Reduced Errors in Protein Folding and Promotion of Normal Protein Function in Proliferation and Apoptosis

Jatropha curcas Linn as a local plant have phytochemical contents, like anti-inflammatory and cytotoxicity properties of phenolic extract. Leaf of J.curcas contents higher phenolic compound, flavonoid and saponin. Application of different varieties of J.curcas in traditional medicine had been reported.

However, information regarding the bioactive compounds and the therapeutic activities is still lacking. These studies suggest that components from J.curcas may have anticancer function and potentially be useful for prevention and treatment strategies.All organisms respond to heat (heat shock response) by upregulating specific heat shock or stress proteins.

Research over the last decade has shown that increased expression of heat shock protein 70 (Hsp70) plays a role in protein folding errors from denaturised proteins and new protein translation, causing proteins to function abnormally. Multiple hits – multiple steps – multiple stage stressors may experience distress and express Hsp70, among other cellular factors, to inhibit cell proliferation and enhance apoptosis.

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Tumoricidal Activation of Macrophages using Jatropha curcas Leaf Extract

Biological activities of the methanolic was under study. Leaf extracts that contains methanolic acid of Jatropha curcas showed the highest antioxidant activity. Other research in cytotoxicity assay results indicated the anticancer therapeutic property of the root extract against human colon adenocarcinoma (HT-29) cell line but its cytotoxic effect on human hepatocyte (Chang cell) was high.

Macrophages can be activated to become neoplasticidal by interaction with a substance filled immune modulators. Neoplasticidal macrophages can recognize and destroy neoplastic cells in vitro and in vivo. Although the exact mechanism by which macrophages discriminate between neoplastic and normal cells is unknown, it is independent of neoplastic cell characteristics such as immunogenicity, metastatic potential, and sensitivity to drugs.

Moreover, macrophage destruction of neoplastic cells apparently is not associated with the development of neoplastic cell resistance. Macrophages are found in association with neoplasm in a definable pattern, suggesting that the most direct way to achieve macrophage-mediated neoplasm regression is in situ macrophage activation.

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