In inflammatory bowel disease (IBD), an inappropriate immune
response leads to chronic mucosal inflammation. This response may be partly due
to dysregulation of defensins, which are endogenously produced antimicrobial
peptides.
This study determined whether microRNAs (miRNAs) regulate
α-defensin 5 (DEFA5), which could further implicate both in IBD
pathogenesis.
Methods: Induction of DEFA5 mRNA and protein expression was determined in
Caco-2 cells.
An in silico analysis identified putative miRNA binding sites
of DEFA5. Expression of these miRNAs was assessed in Caco-2 cells. Regulation
of DEFA5 expression by these miRNAs was measured by luciferase assays. Caco-2
cells were transfected with miR-124 and miR-924 mimics, and DEFA5 mRNA and
protein expression was measured.
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