Sui et al. used a combination of
multiplex-PCR, Illumina sequencing and IMGT/High V-QUEST for a standardized
analysis of the characteristics and polymorphisms of the T-cell receptor β
chain Complementarity-Determining region 3 (TCRβ CDR3) genes in T cells from
Systemic Lupus Erythematosus (SLE) patients and healthy donors (NC). However,
there are several issues which warrant further consideration. Herein, we will
discuss this topic.
It’s honoured to provide a brief
commentary on the brilliant study of Sui et al. concerning the T cell immune
repertoire sequencing (IRSEQ). IR-SEQ refers to a method to evaluate the
diversity of immune system by amplifying
the Complimentary Determining Region (CDR) of B-cell Receptor (BCR ) or T-cell Receptor (TCR )
using multiple-PCR or 5'RACE
methods, followed by high-throughput sequencing, which can be used to
investigate the association between immune repertoire and diseases.
The authors found that there were
more expanded clones and more restricted T-cell repertoire in the SLE group
compared to the NC group. Also, SLE patients showed different usage frequencies
of TRBV and TRBJ segments compared to NC group.
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