Use of suitable
adjuvant is one of the priorities in vaccine and immune modulation, stimulation
and potentiating. Vaccination is effective for prevention and treatment of
bacterial diseases including Leptospirosis. In the present study, we prepared
Leptospiral vaccine with Alum, modified Alum adjuvant (mAlum) and without
adjuvant. Vaccination was done; then we evaluated the immune responses by
isolating the splenocytes and sera for interleukin (IL) profiles (the highest
level of all cytokines except IL-4 and IL-
12 was obtained
in the mAlum antigen group at week 7 post-treatment).
Moreover, the expression
of all evaluated cytokines in the mAlum group was greater than in the other
groups at week 62. Significant increases in antibody titters were noted in the
mAlum and Alum group, challenged interaperitionelly
with a lethal dose of virulent and monitored pathological lesion that moderate to
severe lesions with score 3 were observed in the control group while the
animals immunized with mAlum-antigen and Alum-antigen displayed slight to mild
lesions with an average score of 0.5.
The results
demonstrated that modified Alum Adjuvants are better than Alum adjuvants as
revealed by the enhanced long-term antibody responses.
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